Gene-expression profiling of ZNF217-overexpressing MDA-MB-231 cells

ABSTRACT: To obtain an overview of the cellular functions regulated by ZNF217 signaling in breast-cancer cell lines, we performed global gene-expression profiling on MDA-MB-231-pcDNA6 and MDA-MB-231-ZNF217 cells Two independent cell-culture replicates from the total population of MDA-MB-231-pcDNA6 and MDA-MB-231-ZNF217 stable transfectants were used to generate total RNA. Total RNA was extracted from cell culture using a Qiagen RNA extraction kit and RNA quality was assessed using the BioAnalyzer 2100™ (Agilent Technologies). Complex probes were produced from these RNA, then hybridized to Human genome U133 Plus 2.0 array according to the manufacturer’s recommendations (Affymetrix).

ORGANISM(S): Homo sapiens

SUBMITTER: Julie Vendrell 

PROVIDER: E-GEOD-35511 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress