Role of microRNAs on the pattern of gene expression in the different segments of human epididymis.

ABSTRACT: BACKGROUND: The molecular mechanisms resulting in the establishment of regionalized gene expression in the human epididymis involve numerous and complex regulatory elements that have not yet been fully elucidated. Interestingly, more than 200 microRNAs (miRNAs) have been identified in the human epididymis that could be involved in the regulation of mRNA stability and post-transcriptionnal expression in this organ. METHODS: Using a global miRNA microarray approach, we investigated the correlation between the miRNA signature and the gene expression profile found in three distinct regions (caput, corpus and cauda) of human epididymides from 3 donors. In silico prediction of transcript miRNA targets was performed in silico using TargetScan and Miranda software’s. To experimentally verify some

ORGANISM(S): Homo sapiens

SUBMITTER: Ezequiel Calvo 

PROVIDER: E-GEOD-35522 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress