Mediator promotes CENP-A incorporation at fission yeast centromeres [ChIP-seq]

ABSTRACT: At Schizosaccharomyces pombe centromeres, heterochromatin formation is required for de novo incorporation of the histone H3 variant CENP-A/Cnp1, which in turn directs kinetochore assembly and ultimately chromosome segregation during mitosis. Noncoding RNAs (ncRNAs) transcribed by RNA polymerase II (Pol II) directs heterochromatin formation via the RNAi machinery, but also through RNAiindependent RNA processing factors. Control of centromeric ncRNA transcription is therefore a key factor for proper centromere function. We here use transcriptional profiling, gene inactivation experiments, and chromatin immunoprecipitation analyses to demonstrate that the Mediator complex directs ncRNA transcription and regulates centromeric heterochromatin formation in fission yeast. Mediator co-localizes wi

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Claes Gustafsson 

PROVIDER: E-GEOD-35524 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress