Transcription profiling of mouse model of glaucoma retinal RNA from DBA/2J mice at 3 months before disease onset, and at 8 months, after IOP elevation

ABSTRACT: Purpose: The DBA/2J mouse is a model for secondary angle-closure glaucoma due to iris atrophy and pigment dispersion, which ultimately leads to increased intraocular pressure (IOP). We sought to correlate changes in retinal gene expression with glaucoma-like pathology by performing microarray analysis of retinal RNA from DBA/2J mice at 3 months before disease onset, and at 8 months, after IOP elevation. Methods: IOP was monitored monthly in DBA/2J animals by Tono-Pen and animals with normal (3 months) or elevated IOP (8 months) were identified. RNA was prepared from 3 individual retinas at each age, and the RNA was amplified and used to generate biotin-labeled probe for high density mouse Affymetrix arrays (U430.2). A subset of genes was selected for confirmation by quantitative RT-PCR usi

ORGANISM(S): Mus musculus

SUBMITTER: Monica Vetter 

PROVIDER: E-GEOD-3554 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress