RNA-seq from ENCODE/HudsonAlpha

ABSTRACT: This data was generated by ENCODE. If you have questions about the data, contact the submitting laboratory directly (Florencia Pauli mailto:[e-mail]). If you have questions about the Genome Browser track associated with this data, contact ENCODE (mailto:[e-mail]). This track is produced as part of the ENCODE Project. RNA-seq is a method for mapping and quantifying the transcriptome of any organism that has a genomic DNA sequence assembly (Mortazavi et al., 2008). Biological replicates of ENCODE cell lines were grown on separate culture plates, total RNA was purified and polyA selected two times. mRNA was then fragmented by magnesium-catalyzed hydrolysis, reverse transcribed to cDNA by random priming and amplified. The cDNA was sequenced on an Illumina Genome Analy

ORGANISM(S): Homo sapiens

SUBMITTER: ENCODE DCC 

PROVIDER: E-GEOD-35584 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress