Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNAi knock down of Enigma, a mitochondrial protein with homology to acyl-CoA dehydrogenases, in Drosophila

ABSTRACT: 5 day RNAi treatment to knockdown Enigma, CG9006, a Drosophila mitochondrial protein with homology to acyl-CoA dehydrogenases. Experiment Overall Design: Kc-167 cells, 5 day treatment with Enigma-specific RNAi or beta-lactamase control RNAi. Specifics:1.6X10^6 cells in 0.5mL of serum-free Sang's M3 media incubated for 1 hour with 20ug of dsRNA, subsequently equal volume of M3 media containing 10% serum was added. Cells harvested after 5 days. RNAi efficiency measured by immunoblot for Enigma.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Spyros Artavanis-Tsakonas 

PROVIDER: E-GEOD-3566 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Enigma, a mitochondrial protein affecting lifespan and oxidative stress response in Drosophila.

Mourikis Philippos P   Hurlbut Gregory D GD   Artavanis-Tsakonas Spyros S  

Proceedings of the National Academy of Sciences of the United States of America 20060124 5

Deregulation of energy metabolism by external interventions or mutations in metabolic genes can extend lifespan in a wide range of species. We describe mutations in Drosophila melanogaster that confer resistance to oxidative stress and display a longevity phenotype. These phenotypes are associated with molecular lesions in a hitherto uncharacterized gene we named Enigma. We show that Enigma encodes a mitochondrial protein with homology to enzymes of the beta-oxidation of fatty acids and that mut  ...[more]

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