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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Analysis of H3K79 methylation during reprogramming

ABSTRACT: Chromatin immunoprecipitation followed by Solexa sequencing for H3K27me3 and H3K79me2 in Fibroblasts, Embryonic stem cells, and fibroblast undergoing reprogramming Inhibition of the histone 3 lysine 79 (H3K79) methyltransferase increases repgoramming efficiency and genome-wide analysis of H3K79me2 distribution revealed that fibroblast-specific genes associated with the epithelial to mesenchymal transition lose H3K79me2 in the initial phases of reprogramming. Dot1L inhibition facilitates the loss of this mark from genes that are fated to be repressed in the pluripotent state.

ORGANISM(S): Homo sapiens

SUBMITTER: Amit Sinha

PROVIDER: E-GEOD-35791 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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H3K79me2 ChIP-seq in mouse proximal intestinal Lgr5(hi) stem cells and villus cells

Project description:H3K79me2 ChIP-seq in mouse proximal intestinal Lgr5(hi) stem cells and villus cells Examination of H3K79me2 modifications between Lgr5(hi) stem cells and differentiated villus cells

2012-10-13 | E-GEOD-41542 | biostudies-arrayexpress

H3K79 methylation - cell cycle

Project description:Mammalian DNA replication starts at distinct chromosomal sites in a tissue-specific pattern coordinated with transcription, but previous studies have not yet identified a chromatin modification that correlates with the initiation of DNA replication. This submission is associated with a paper in which we report that replication initiation events are associated with a high frequency of methylation of histone H3 on lysine K79 (H3K79Me2 and H3K79Me3). H3K79Me2-containing chromatin exhibited the highest enrichment of replication initiation events observed in a single chromatin modification. Importantly, H3K79 methylation was enriched in chromatin containing a replicator (a DNA sequence capable of initiating DNA replication), but not in chromatin containing a mutant replicator that could not initiate replication. The association of H3K79Me2 with replication initiation sites was independent and not synergistic with other chromatin modifications. H3K79 methylation exhibited a wider distribution and greater abundance during S-phase, but regions of chromatin that were only modified during S-phase were not enriched in replication initiation events. In addition, the paper shows that depletion of DOT1L, the sole enzyme responsible for H3K79 methylation, triggered limited genomic over-replication. These data are consistent with the hypothesis that methylation of H3K79 associates with replication origins and marks replicated chromatin during S-phase to prevent re-replication and preserve genomic stability. Evaluation of HeK79 methylation in chromatin samples from cell cycle fractionated K562 leukemia cells. Unsyncrhonized untreated cultures of K562 cells were fractionated by size using centrifugal elutriation. Chromatin was isolated and subject to ChIP-Seq with antibodies directed against dimethylated and trimethylated lysine on histone H3.

2013-06-06 | E-GEOD-35294 | biostudies-arrayexpress

Analysis of H3K79 methylation during reprogramming

Project description:Chromatin immunoprecipitation followed by Solexa sequencing for H3K27me3 and H3K79me2 in Fibroblasts, Embryonic stem cells, and fibroblast undergoing reprogramming

2012-02-22 | GSE35791 | GEO

The Histone Methyltransferases MLL1 and DOT1L Cooperate with Meningioma-1 to Induce AML [Mouse ChIP-seq]

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2011-09-13 | E-GEOD-29130 | biostudies-arrayexpress

Degree of Recruitment of DOT1L to MLL-AF9 Defines Level of H3K79 Di- and Tri-methylation on Target Genes and Transformation Potential

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2015-04-30 | E-GEOD-64365 | biostudies-arrayexpress

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2010-11-02 | E-GEOD-23784 | biostudies-arrayexpress

Nucleosome positioning in the IMR90 cell line

Project description:Micrococcal nuclease (Mnase) treatment and sequencing of mononucleosomal DNA One single sample in the IMR90 fibroblast cell line

2015-03-03 | E-GEOD-44985 | biostudies-arrayexpress

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Project description:Reprogramming of the human fibroblast genome into the state of the hematopoietic progenitors Two samples analyzed.

2011-08-03 | E-GEOD-23414 | biostudies-arrayexpress

ChIP-Seq of Histone Methylation and RNAP2 in Human Jurkat cells.

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2010-07-24 | E-GEOD-23080 | biostudies-arrayexpress

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