Wild-type versus Pumilio mutant flies
Ontology highlight
ABSTRACT:
ORGANISM(S): Drosophila melanogaster
SUBMITTER: Andre Gerber
PROVIDER: E-GEOD-3580 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Genome-wide identification of mRNAs associated with the translational regulator PUMILIO in Drosophila melanogaster.
Gerber André P AP Luschnig Stefan S Krasnow Mark A MA Brown Patrick O PO Herschlag Daniel D
Proceedings of the National Academy of Sciences of the United States of America 20060313 12
Genome-wide identification of RNAs associated with RNA-binding proteins is crucial for deciphering posttranscriptional regulatory systems. PUMILIO is a member of the evolutionary conserved Puf-family of RNA-binding proteins that repress gene expression posttranscriptionally. We generated transgenic flies expressing affinity-tagged PUMILIO under the control of an ovary-specific promoter, and we purified PUMILIO from whole adult flies and embryos and analyzed associated mRNAs by using DNA microarr ...[more]
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