Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Comparison of hypoxia (4 % O2) cultured human embryonic stem cells (hESCs) to normoxia (21 % O2) cultured

ABSTRACT: We compared the transcriptome at gene expression level in hypoxic and normoxic conditions. The transcriptome of cells cultured at three different timepoints, 2h, 24 h and 7 days with three different cell lines in normoxia and hypoxia was compared 18 samples total, 3 replicates in each conditions, 2h, 24 h and 7day timepoints in hypoxia and normoxia

ORGANISM(S): Homo sapiens

SUBMITTER: Elisa Närvä 

PROVIDER: E-GEOD-35819 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Low oxygen tension (hypoxia) contributes critically to pluripotency of human embryonic stem cells (hESCs) by preventing spontaneous differentiation and supporting self-renewal. However, it is not well understood how hESCs respond to reduced oxygen availability and what are the molecular mechanisms maintaining pluripotency in these conditions. In this study we characterized the transcriptional and molecular responses of three hESC lines (H9, HS401 and HS360) on short (2 hours), intermediate (24 h  ...[more]