Transcription map of the Swine Leukocyte Antigen complex and comparative biology of MHCs among vertebrates

ABSTRACT: Tiling arrays have been reported as highly relevant tools to improve annotation and identify new transcripts in genome sequences. Our aim was to refine annotation of the Swine Leucocyte Antigen (SLA) complex, which is a gene dense region spanning 2.4 megabases on both sides of the centromere of chromosome 7 with many genes involved in immune response and inflammation. To date, one hundred and fifty-one SLA genes and pseudogenes have been characterized or predicted. A high-density tiling array was produced, which covered the entire Hp1a.0 haplotype sequence and partially the centromeric junction on the q-arm. The array contained 386,620 55-70-mer oligonucleotides designed on both strands and tiled on average every nine bases. It was used to compare the transcriptome of peripheral blood mono

ORGANISM(S): Sus scrofa

SUBMITTER: Yu GAO 

PROVIDER: E-GEOD-35828 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress