Real-time transcriptional profiling of cellular and viral gene expression during lytic cytomegalovirus infection

ABSTRACT: During viral infections cellular gene expression is subject to rapid alterations induced by both viral and antiviral mechanisms. In this study, we applied metabolic labeling of newly transcribed RNA with 4-thiouridine (4sU-tagging) to dissect the real-time kinetics of cellular and viral transcriptional activity during lytic murine cytomegalovirus (MCMV) infection. Microarray profiling on newly transcribed RNA obtained from different times during the first six hours of MCMV infection revealed discrete functional networks of cellular genes regulated with distinct kinetics at surprising temporal resolution. Most of these were undetectable in total RNA, either due to low temporal sensitivity of standard gene expression profiling using total RNA, or due to rapid (viral) counter-regulation. Furt

ORGANISM(S): Mus musculus

SUBMITTER: Lukas Windhager 

PROVIDER: E-GEOD-35833 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress