Transcriptional analysis of prebiotic uptake and catabolism by Lactobacillus acidophilus NCFM

ABSTRACT: The global transcriptome of the wild type Lactobacillus acidophilus NCFM strain (NCK56) was measured during exponential growth on 11 prebiotic carbohydrates and glucose to identify the specific gene cluster differentially upregulated in response to each carbohydrate. Oligoarray hybridization experiments were performed to compare the differential transcriptional profiles of the NCK56 at the early-log (OD600nm 0.3-0.5) phase when cultured in semi-synthetic medium (SSM, Barrangou et al., 2003 PNAS. 100:8957-8962, supplemented with a range of 1% (w/v) carbohydrates, at 37 M-BM-0C and harvested by centrifugation and flash freezing. A total of 12 NCK56 cultures were prepared by growing in SSM supplemented with each one of the 12 tested carbohydrates. Each culture was grown in fresh carbohyd

ORGANISM(S): Lactobacillus acidophilus NCFM

SUBMITTER: Todd Klaenhammer 

PROVIDER: E-GEOD-35968 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress