Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Transcription profiling of mouse follicular and marginal zone B-cells

ABSTRACT: Immature B cells in spleens of mouse and human differentiate into at least two subsets of mature B cells, the follicular (FO) B cells and the marginal zone (MZ) B cells, but functions, maturation and other properties of these cells are largely unknown. To solve these questions, in this study, we performed transcriptome analyses of FO and MZ B cells sorted from spleens of normal unimmunized mice using gene chips. By comparison of gene expression profiles between FO and MZ B cells, we identified 1226 genes that are expressed higher in FO B cells than in MZ B cells. On the other hand, 1734 genes were found to be expressed higher in MZ B cells than in FO B cells. We noticed that some of differentially expressed genes have been reportedly characterized in FO and MZ B cells, suggesting the reliability of the analysis. By using FACS analyses, we confirmed that CD36, CD68, and CD49e are expressed on MZ B-cells but not on FO B-cells. These results revealed new phenotypic and functional properties of FO and MZ B cells, and set a molecular basis for further studying differentiation and functions of these mature B cells. Experiment Overall Design: one follicular B cells replicate and one marginal zone B cells replicate were analyzed.

ORGANISM(S): Mus musculus

SUBMITTER: ping zhang 

PROVIDER: E-GEOD-3599 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Identification of CD36 as a new surface marker of marginal zone B cells by transcriptomic analysis.

Zhang Ping P   Li Wei W   Wang Yaochun Y   Hou Lihong L   Xing Ying Y   Qin Hongyan H   Wang Jishu J   Liang Yingmin Y   Han Hua H  

Molecular immunology 20060417 4

Follicular (FO) B cells and marginal zone (MZ) B cells belong to the mature B cell population in spleen of mice. To identify new surface markers of these mature B cell subsets, we compared gene expression profiles of FO and MZ B cells by DNA microarray using FACS-sorted mouse FO and MZ B cells. From 14,000 mouse genes, 27 membrane proteins were expressed mainly in MZ B cells while another 22 membrane proteins expressed largely in FO B cells. Using FACS analysis, we identified that CD36, CD68, an  ...[more]

Similar Datasets

Transcriptomics Programming of marginal zone B cell fate by basic krüppel-like factor (BKLF/KLF3)
2011-01-29 | E-GEOD-26948 | biostudies-arrayexpress
Transcriptomics Gene expression profiling of spleen marginal zone B cells and spleen follicular B cells in IRF8 conditional KO mice
2010-12-20 | E-GEOD-24972 | biostudies-arrayexpress
Unknown Comparison of gene expression profiles between mouse follicular and marginal zone B-cells
2006-04-21 | GSE3599 | GEO
Genomics Transcription factor Ebf1 regulates differentiation stage-specific signaling, proliferative expansion and survival of B cells [H3K4me2 and H3K4me3]
2012-04-02 | E-GEOD-35914 | biostudies-arrayexpress
Unknown Transcriptomic analyis of donor (B6:SJL.C20) and host follicular and marginal zone B cells after adoptive transfer of follicular B cells into into lymphopenic (Rag2-/-) or replete animals (C57BL6)
2022-05-01 | GSE200219 | GEO
Proteomics The proteome of B cell maturation identifies PDCD4 as limiting the response of marginal zone B cells – marginal zone B cells WT and PDCD4 KO
2023-06-21 | PXD037563 | Pride
Proteomics The proteome of B cell maturation identifies PDCD4 as limiting the response of marginal zone B cells – WT and PDCD4 KO MZ B cells. Includes Fasta file
2023-06-29 | PXD043351 | Pride
Genomics Transcription factor Ebf1 regulates differentiation stage-specific signaling, proliferative expansion and survival of B cells
2012-04-02 | E-GEOD-35915 | biostudies-arrayexpress
Unknown Gene expression profiling of spleen marginal zone B cells and spleen follicular B cells in IRF8 conditional KO mice
2010-12-20 | GSE24972 | GEO
Transcriptomics Gene expression analysis of wild type and KAP1 KO splenic B cells
2012-05-16 | E-GEOD-34446 | biostudies-arrayexpress