Genome-wide gene expression analysis of the whole blood leukocyte transcriptional response to endotoxin treatment

ABSTRACT: Several inflammatory diseases respond to TNFα inhibitors, implicating TNFα signaling in the pathogenesis of these conditions. Here, we set out to map the systemic genome-wide transcriptome influenced by TNFα release. We performed an intravenous endotoxin challenge in 16 healthy subjects, half of whom were pretreated with the soluble TNF receptor fusion protein, etanercept. Whole-blood leukocyte total RNA was isolated using the PAXgeneTM tube and PAXgeneTM RNA isolation system (PreAnalytiXTM, Qiagen) as described by the manufacturer. Total RNA yield and purity (260nm:280nm) were determined spectrophotometrically. The integrity of the re-suspended total RNA was determined using the RNA Nano Chip Kit on the Bioanalyzer 2100 and the 2100 Expert software (Agilent). To increase the sensitivity o

ORGANISM(S): Homo sapiens

SUBMITTER: Brendon Scicluna 

PROVIDER: E-GEOD-36177 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress