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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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PAR-CLIP of RBM4 and HIF-2a

ABSTRACT: To identify which mRNAs bind to RBM4/HIF-2a Two PAR-CLIPs were performed: One of an RBM4 immunoprecipitation, and the other of a HIF-2a immunoprecipitation and excising the associated RBM4 band.

ORGANISM(S): Homo sapiens

SUBMITTER: Jim Uniacke

PROVIDER: E-GEOD-36247 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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An oxygen-regulated switch in the protein synthesis machinery.

Uniacke James J Holterman Chet E CE Lachance Gabriel G Franovic Aleksandra A Jacob Mathieu D MD Fabian Marc R MR Payette Josianne J Holcik Martin M Pause Arnim A Lee Stephen S

Nature 20120506 7401

Protein synthesis involves the translation of ribonucleic acid information into proteins, the building blocks of life. The initial step of protein synthesis is the binding of the eukaryotic translation initiation factor 4E (eIF4E) to the 7-methylguanosine (m(7)-GpppG) 5' cap of messenger RNAs. Low oxygen tension (hypoxia) represses cap-mediated translation by sequestering eIF4E through mammalian target of rapamycin (mTOR)-dependent mechanisms. Although the internal ribosome entry site is an alte ...[more]

PMID: 22678294

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