Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Effects of neonatal dosing of SERMs on CD1 mouse uterine gene expression at 3 months


ABSTRACT: Tamoxifen, a selective estrogen receptor modulator (SERM), is an effective treatment for breast cancers. In the CD-1 mouse model, neonatal oral dosing with tamoxifen leads to the development of adenomyosis. Both 4-hydroxyestradiol and tamoxifen can form DNA-reactive metabolites and may be involved in carcinogenesis of the uterus. After comparing the uterotrophic response of several SERMs the maximal uterotrophic doses of estradiol (100mg/kg) 4-hydroxyestradiol (385mg/kg) and tamoxifen (250mg/kg) were determined. Maximal uterotrophic doses were given orally to newborn CD-1 mice on days 1 – 4 after birth and gene and pathological changes examined in the uterus at 3 months after dosing. ERKOa knockout mice were dosed orally with tamoxifen (1mg/Kg) on days 1 – 4 after birth and uterine gene expression compared with CD-1 mice. Dosing groups: Estradiol (E2): 100mg/Kg 4-hydroxyestradiol (4OHE2): 385mg/Kg Tamoxifen : 250mg/Kg ERKO mice dosed with tamoxifen (1mg/Kg) 4 dosed animals and 4 controls for each treatment group. Uteri removed at 3 months after dosing and total RNA extracted. Controls were pooled. RNA labelling, hybridisation and analysis of fluorescence was carried out as described by Turton et al (2001). Cy3/Cy5 dye swap labelling was carried out on samples from each animal. Reference: Turton NJ et. al. (Oncogene (2001) 20, 1300-1306

ORGANISM(S): Mus musculus

SUBMITTER: Jerry Styles 

PROVIDER: E-GEOD-3625 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2010-06-30 | E-GEOD-3626 | biostudies-arrayexpress
2010-07-01 | E-GEOD-3762 | biostudies-arrayexpress
2010-06-09 | E-GEOD-2419 | biostudies-arrayexpress
2009-08-31 | E-GEOD-17070 | biostudies-arrayexpress
2010-06-24 | E-GEOD-13560 | biostudies-arrayexpress
2010-06-09 | E-GEOD-2420 | biostudies-arrayexpress
2011-10-31 | E-GEOD-33269 | biostudies-arrayexpress
2010-05-27 | E-GEOD-2069 | biostudies-arrayexpress
2004-07-28 | E-GEOD-1592 | biostudies-arrayexpress
2010-05-26 | E-GEOD-1545 | biostudies-arrayexpress