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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Genome-wide analysis of gene expression in human embryonic stem cells (hESCs) and their differentiated cells

ABSTRACT: In order to investigate the gene expression changes in human embryonic stem cells (hESCs) during differentiation, we performed a microarray analysis from RNAs isolated from undifferentiated hESCs and their differentiated cells incubated for 1 week or 2 weeks in ESC medium. Human SNUhES3 ESCs (Seoul National University Hospital, Seoul, Korea) were cultured on mitotically-arrested STO feeder cells (ATCC, Manassas, USA) in DMEM/F12 supplemented with 20% knockout serum replacement (KSR). The embryoid bodies were incubated for 1 or 2 weeks in the above ESC medium without bFGF. Cells were then lysed and RNA was isolated.

ORGANISM(S): Homo sapiens

SUBMITTER: Hyobean Jeong

PROVIDER: E-GEOD-36255 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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