Enhancer Invasion and Transcriptional Amplification in Tumor Cells with Elevated c-Myc

ABSTRACT: Excessive expression of c-Myc occurs frequently in human cancers, where high levels are associated with tumor aggression and poor clinical outcome, but the effect of high levels of c-Myc on global gene regulation is poorly understood. We report here that in tumor cells expressing high levels of c-Myc, the transcription factor binds to E-box sequences in the core promoters of most actively transcribed genes and, unexpectedly, the enhancers of these active genes. The predominant effect of increasing c-Myc levels at both proximal and distal promoter elements is to produce higher levels of transcription at existing active genes by promoting RNA polymerase II elongation, as opposed to stimulating transcription of novel target genes. Our results argue that c-Myc overexpression drives increased

ORGANISM(S): Homo sapiens

SUBMITTER: Richard Young 

PROVIDER: E-GEOD-36354 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress