Efficient Generation of Integration-free and Engraftable induced Neural Stem Cells (iNSC) from Epithelial-like Cells in Human Urine

ABSTRACT: Human neural stem cells (NSCs) hold great promise in reparative therapy for neural diseases and injuries. However, the isolation of NSCs from either fetal or adult tissue and their application remain difficult due to ethical and immune-rejection concerns. One promising solution is to generate patient specific induced pluripotent stem cells (iPSCs) and subsequently differentiate them into NSCs. Alternatively, induced neurons (iN) can be generated directly from fibroblasts by retroviral delivery of neural specific transcription factors or miroRNAs. The later two approaches remain inefficient and with safety concerns. Here, we describe a process to generate, from epithelial-like cells present in human urine, integration free and engraftable NSCs (UiNSC) efficiently. Using an episomal syst

ORGANISM(S): Homo sapiens

SUBMITTER: Guangjin Pan 

PROVIDER: E-GEOD-36395 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress