The CCR4-NOT Complex Is Implicated in the Viability of Aneuploid Yeasts

ABSTRACT: To identify the genes required to sustain aneuploid viability, we screened a deletion library of non-essential genes in the fission yeast Schizosaccharomyces pombe, in which most types of aneuploidy are eventually lethal to the cell. Aneuploids remain viable for a period time and can form colonies by reducing the extent of the aneuploidy. We hypothesized that a reduction in colony formation efficiency could be used to screen for gene deletions that compromise aneuploid viability. Deletion mutants were used to measure the effects on the viability of spores derived from triploid meiosis and from a chromosome instability mutant. We found that the CCR4-NOT complex, an evolutionarily conserved general regulator of mRNA turnover, and other related factors, including poly(A)-specific nuclease

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Yuji Chikashige 

PROVIDER: E-GEOD-36454 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress