Reprogramming of two somatic nuclei in the same ooplasm leads to pluripotent embryonic stem cells

ABSTRACT: We provide proof that the oocyte is able to reprogram two somatic nuclei at once and therefore is not limited in its reprogramming capacity to a single nucleus. Mouse ooplasts transplanted with 2 somatic cell nuclei simultaneously (double nuclear transfer) support preimplantation development and the derivation of tetraploid NT embryonic stem cells (tNT-ES cells). These cells are unique in that they embody the first case of cloned ES cells containing two reprogrammed somatic genomes at the same time and exhibit characteristics of true pluripotency. By assessing allelic expression of Oct4 in normal and double nucleus transplanted embryos throughout development we demonstrate that oocyte mediated reprogramming is embarked with a probabilistic component and that one reprogrammed genome can dom

ORGANISM(S): Mus musculus

SUBMITTER: Marcos Araúzo-Bravo 

PROVIDER: E-GEOD-36468 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress