Direct conversion of fibroblasts into stably expandable neural stem cells

ABSTRACT: Recent advances have suggested that direct induction of neural stem cells could provide an alternative to derivation from somatic tissues or pluripotent cells. Here we show direct derivation of stably expandable NS cells from mouse fibroblasts through a curtailed version of reprogramming to pluripotency. By constitutively inducing Sox2, Klf4, and c-Myc while strictly limiting Oct4 activity to the initial phase of reprogramming, we generated neurosphere-like colonies that could be expanded for more than 50 passages and do not depend on sustained expression of the reprogramming factors. These induced NS (iNS) cells uniformly display morphological and molecular features of NS cells such as the expression of Nestin, Pax6, and Olig2 and have a similar genome-wide transcriptional profile to brai

ORGANISM(S): Mus musculus

SUBMITTER: Frank Edenhofer 

PROVIDER: E-GEOD-36484 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress