Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of mouse Anterior interpositus nucleus centred DCN from mice with of delayed-type eyeblink conditioned mice: 3-d paired training and sham negative control groups

ABSTRACT: Anterior interpositus nucleus (AIN) is a proposed site of memory formation of eyeblink conditioning. A large part of the underlying molecular events, however, remains unknown. To elucidate molecular mechanisms, we examined transcriptional changes in the AIN of mice trained with delayed-type eyeblink conditioning Experiment Overall Design: 3-d paired training group: Mice received a surgery for implanting four Teflon-coated stainless-steel wires in their left eyelid and a headstage on their head. After 3daysâ?? recovery, they were trained with paired paradigm of conditioned stimulus (CS) and unconditioned stimulus (US) for 3days: A 352-ms tone CS (1kHz, 83~85dB) was delivered first and a 100ms periorbital shock US (100kHz square pluses) were delivered with 252ms after the onset of CS, and they co-terminated. After the last trial was given, anterior interpositus was immediately sampled in 10 to 30 min from the sacrifice. Experiment Overall Design: Sham negative control group: Mice received a surgery for implanting four Teflon-coated stainless-steel wires in their left eyelid and a headstage on their head. After recovery, anterior interpositus nucleus-centered deep cerebellar nuclei were immediately sampled in 10 to 30 min from the sacrifice. Experiment Overall Design: ~10-15 anterior interpositus-centered deep cerebellar nuclei ipsilateral to the eye implanted with four wires were pooled into the same sham control or training group and subjected to microarray analysis.

ORGANISM(S): Mus musculus

SUBMITTER: Jin-Sung Park

PROVIDER: E-GEOD-3651 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Molecular evidence for two-stage learning and partial laterality in eyeblink conditioning of mice.

Park Jin-Sung JS Onodera Takashi T Nishimura Shin-ichi S Thompson Richard F RF Itohara Shigeyoshi S

Proceedings of the National Academy of Sciences of the United States of America 20060328 14

The anterior interpositus nucleus (AIN) is the proposed site of memory formation of eyeblink conditioning. A large part of the underlying molecular events, however, remain unknown. To elucidate the molecular mechanisms, we examined transcriptional changes in the AIN of mice trained with delay eyeblink conditioning using microarray, quantitative real-time RT-PCR, and in situ hybridization techniques. Microarray analyses suggested that transcriptionally up-regulated gene sets were largely differen ...[more]

PMID: 16569693

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Dataset Information

Transcription profiling of mouse Anterior interpositus nucleus centred DCN from mice with of delayed-type eyeblink conditioned mice: 3-d paired training and sham negative control groups

Publications

Molecular evidence for two-stage learning and partial laterality in eyeblink conditioning of mice.

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