Protein Arginine Methyltransferase 6 dependent gene expression and splicing: Association with breast cancer outcomes

ABSTRACT: Protein arginine methyltransferase-6 (PRMT6) regulates steroid-dependent transcription and alternative splicing, and is implicated in endocrine system development and function, cell death, cell cycle, gene expression and cancer. Despite its role in these processes, little is known about its function and cellular targets in breast cancer. To identify novel gene targets regulated by PRMT6 in breast cancer cells, we used a combination of small interfering RNA (siRNA) and exon-specific microarray profiling in vitro, coupled to in vivo validation in normal breast and primary human breast tumours. This approach, which allows the examination of genome-wide changes in individual exon usage and total transcript levels, demonstrated PRMT6 knockdown significantly affected: (i) the transcription of 15

ORGANISM(S): Homo sapiens

SUBMITTER: Michael Pearen 

PROVIDER: E-GEOD-36542 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress