Tracing pluripotency of human early embryos and embryonic stem cells by single cell RNA-seq

ABSTRACT: Find the casual relationship between gene expression network and cellular phenotype at single cell resolution. We collected donated human pre-implatation embryos, and the embryonic stem cells derived from them, isolate individual cells, prepared single cell cDNAs, and sequenced them by HiSeq2000. Then we analyzed the expression of known RefSeq genes. We get transcriptome of 124 individual cells from human pre-implantation embryos and human embryonic stem cells by applying single cell RNA-seq technique we recently developed[1][2][3][4]. We did in-depth bioinformatic analysis to these data and found very dynamic expression of protein-coding genes. [1] Tang, F. et al. (2010a) Tracing the Derivation of Embryonic Stem Cells from the Inner Cell Mass by Single-Cell RNA-Seq Analysis. Cell Stem Cel

ORGANISM(S): Homo sapiens

SUBMITTER: Mingyu Yang 

PROVIDER: E-GEOD-36552 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress