Genome-wide map of transcription factor GATA1 occupancy during mitosis in G1E ER4+E2 cells

ABSTRACT: Tissue-specific transcription patterns are preserved throughout cell divisions to maintain lineage fidelity. We investigated whether transcription factor GATA1 plays a role in transmitting hematopoietic gene expression programs through mitosis when transcription is transiently silenced. Live cell imaging revealed that a fraction of GATA1 is retained focally within mitotic chromatin. ChIP-seq of highly purified mitotic cells uncovered that key hematopoietic regulatory genes are occupied by GATA1 in mitosis. The GATA1 co- regulators FOG1 and TAL1 dissociate from mitotic chromatin, suggesting that GATA1 functions as platform for their postmitotic recruitment. Mitotic GATA1 target genes tend to re-activate more rapidly upon entry into G1 than genes from which GATA1 dissociates. A novel system

ORGANISM(S): Mus musculus

SUBMITTER: Ross Hardison 

PROVIDER: E-GEOD-36589 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress