Gene Regulation by Estrogen Signaling and DNA Methylation in MCF7 Breast Cancer Cells

ABSTRACT: Estrogen signaling and epigenetic modifications, in particular DNA methylation, are involved in regulation of gene expression in breast cancers. Here we investigated a potential regulatory cross-talk between these two pathways by identifying their common target genes and exploring potential underlying molecular mechanisms in human MCF7 breast cancer cells. Principal Findings: Gene expression profiling revealed that the expression of approximately 150 genes was influenced by both 17β-estradiol (E2) and a hypomethylating agent 5-aza-2’-deoxycytidine (DAC). Based on gene ontology (GO), CpG island prediction analysis and previously reported estrogen receptor (ER) binding regions, we selected six genes for further analysis (BTG3, FHL2, PMAIP1, BTG2, CDKN1A and TGFB2). GO analysis suggests that

ORGANISM(S): Homo sapiens

SUBMITTER: Milica Putnik 

PROVIDER: E-GEOD-36683 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress