Glioma-propagating cells as an in vitro screening platform: PLK1 as a case study

ABSTRACT: Gliomas are the most devastating of primary adult malignant brain tumors. These tumors are highly infiltrative and can arise from cells with extensive self-renewal capability and chemoresistance, frequently termed glioma-propagating cells (GPCs). GPCs are thus the plausible culprits of tumor recurrence. Treatment strategies that eradicate GPCs will greatly improve disease outcome. Such findings support the use of GPCs as in vitro cellular systems for small molecule screening. However, the nuances in utilizing GPCs as a cellular screening platform are not trivial. These slow-growing cells are typically cultured as suspension, spheroid structures in serum-free condition supplemented with growth factors. Consequently, replenishment of growth factors throughout the screening period must occur

ORGANISM(S): Homo sapiens

SUBMITTER: Carol Tang 

PROVIDER: E-GEOD-36782 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress