Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression profile of PfmR (TTHB023)-deficient Thermus thermophilus HB8 strain


ABSTRACT: In order to find function of PfmR protein from T. thermophilus HB8, we observed the expression profile of the total mRNA of the PfmR-deficient strain grown in a rich medium at 70M-BM-0C for 360 min. Three PfmR-deficient T. thermophilus HB8 strains were each pre-cultured at 70M-BM-0C for 16 h in 4 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (1.6 ml) were inoculated into 1 liter of the same medium and then cultivated at 70M-BM-0C. Cells were collected after 360 min, and then, the expression of each mRNA was analyzed.

ORGANISM(S): Thermus thermophilus HB8

SUBMITTER: Akeo Shinkai 

PROVIDER: E-GEOD-36881 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Transcriptional repression mediated by a TetR family protein, PfmR, from Thermus thermophilus HB8.

Agari Yoshihiro Y   Sakamoto Keiko K   Kuramitsu Seiki S   Shinkai Akeo A  

Journal of bacteriology 20120629 17


PfmR is one of four TetR family transcriptional regulators found in the extremely thermophilic bacterium, Thermus thermophilus HB8. We identified three promoters with strong negative regulation by PfmR, both in vivo and in vitro. PfmR binds pseudopalindromic sequences, with the consensus sequence of 5'-TACCGACCGNTNGGTN-3' surrounding the promoters. According to the amino acid sequence and three-dimensional structure analyses of the PfmR-regulated gene products, they are predicted to be involved  ...[more]

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