Ten percent of aberrant cells is sufficient for detection of DNA copy number alterations (part 2)

ABSTRACT: Array Comparative Genomic Hybridization (aCGH) is a widely used technique to assess chromosomal copy number alterations. Chromosomal content, however, is often not uniform throughout cell populations. The aim of our present study is to evaluate to what extent aCGH can detect DNA copy number alterations in a heterogeneous cell population. Reported detection limits are a compound of analytical software and laboratory technique whilst systematic evaluation is lacking, despite the importance in diagnostics and research. Detection limits were explored with DNA isolated from a patient with intellectual disability (ID) and from tumor cell line BT474. Both were diluted with increasing amounts of normal DNA to simulate different levels of cellularity. Samples were hybridized on CGH arrays containi

ORGANISM(S): Homo sapiens

SUBMITTER: Oscar Krijgsman 

PROVIDER: E-GEOD-36986 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress