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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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PGE2-induced OSM expression

ABSTRACT: The objective of the study was to compare the wound macs with corresponding macs derived from peripheral blood monocytes (MDMs). Wound site macrophage (wound macs were isolated from human subjects with chronic wounds. Matching blood monocyte derived macrophages (MDM) were obtained from same subjects. Transcriptome profiling (GeneChip, Affymetrix) was performed.The expression values of genes were normalized using global scaling approach. Blood monocyte derived macrophages or human wound macrophages were isolated and transcriptome analysis was performed using affymetrix gene chip analysis. Group -1 MDMs (n=3) Mac-1 Mac-2 Mac-3 Group -2 Wound macs (n=3) Wmac-1 Wmac-2 Wmac-3

ORGANISM(S): Homo sapiens

SUBMITTER: Sashwati Roy

PROVIDER: E-GEOD-36995 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Prostaglandin E₂ induces oncostatin M expression in human chronic wound macrophages through Axl receptor tyrosine kinase pathway.

Ganesh Kasturi K Das Amitava A Dickerson Ryan R Khanna Savita S Parinandi Narasimham L NL Gordillo Gayle M GM Sen Chandan K CK Roy Sashwati S

Journal of immunology (Baltimore, Md. : 1950) 20120727 5

Monocytes and macrophages (m) are plastic cells whose functions are governed by microenvironmental cues. Wound fluid bathing the wound tissue reflects the wound microenvironment. Current literature on wound inflammation is primarily based on the study of blood monocyte-derived macrophages, cells that have never been exposed to the wound microenvironment. We sought to compare pair-matched monocyte-derived macrophages with m isolated from chronic wounds of patients. Oncostatin M (OSM) was differen ...[more]

PMID: 22844123

Similar Datasets

PGE2-induced OSM expression

Project description:The objective of the study was to compare the wound macs with corresponding macs derived from peripheral blood monocytes (MDMs). Wound site macrophage (wound macs were isolated from human subjects with chronic wounds. Matching blood monocyte derived macrophages (MDM) were obtained from same subjects. Transcriptome profiling (GeneChip, Affymetrix) was performed.The expression values of genes were normalized using global scaling approach.

2012-08-02 | GSE36995 | GEO

Affymetrix microarray of human monocyte derived macrophages following challenge with Streptococcus pneumoniae with or without pneumolysin

Project description:Transcriptional profiling of primary monocyte derived macrophages (MDMs) comparing control untreated MDMs with MDMs exposed with Serotype 2 Streptococcus pneumoniae (D39) strain (MOI 10) for 3 hours) Three-condition experiment, control MDMs vs. D39 infected MDMs vs Stop infected MDMs. Biological replicates: 3 control replicates, 3 infected D39 replicates and 3 infected penumolysin deficient (STOP) MOI 10.

2021-03-01 | E-MTAB-9055 | biostudies-arrayexpress

cART Treated monocyte derived macrophages

Project description:primary human monocyte derived macrophages (MDMs) treated with antiretrovirals, specifically Atripla (A) or Triumeq (T)

2022-02-28 | GSE195708 | GEO

Mycobacterium avium subsp. paratuberculosis (MAP) during bovine monocyte-derived macrophage infection: untreated vs. bafilomcyin A1 treatment

Project description:Transcriptional profiling of M. avium subsp. paratuberculosis inside bovine monocyte-derived macrophages (MDMs; in vitro infection) during phagosome acidification at 30 min post-infection. M. avium subsp. paratuberculosis profiles from bafilomcyin A1 (acidification inhibitor) treated and untreated monocyte-derived macrophages were compared.

2016-07-29 | GSE84708 | GEO

Transcription profiling of human primary macrophages experiencing hypoxia reveals hypoxia inducible factors 1 and 2 are important transcriptional effectors

Project description:Ischemia exists in many diseased tissues including arthritic joints, atherosclerotic plaques and malignant tumors. Macrophages accumulate in these sites and upregulate genes in response to the hypoxia present. We used microarrays to detail the hypoxia upregulated gene in human primary macrophages. Experiment Overall Design: Primary macrophages were differentiated for 7 days in vitro from human peripheral blood (monocyte-derived macrophages, MDMs) and were subjected to severe hypoxia (< 0.5% O2) or normoxia (20.9% O2) in 5% CO2 humidified multi-gas incubators.

2009-05-11 | E-GEOD-15949 | biostudies-arrayexpress

Mycobacterium avium subsp. paratuberculosis (MAP) during bovine monocyte-derived macrophage infection: untreated vs. bafilomcyin A1 treatment

2016-07-29 | E-GEOD-84708 | biostudies-arrayexpress

Transcription profiling of human monocyte-derived macrophages infected in vitro with wild type Leishmania chagasi parasites and then cultured with naive, autologous T-cells

Project description:We performed gene expression analysis using Affymetrix U133Plus2 microarray chips on cultures of infected human cells. To mimic an in vivo infection, we infected human monocyte-derived macrophages (MDMs) in vitro with wild-type L. chagasi parasites and then co-cultured those infected MDMs with naive, autologous T-cells.

2007-11-01 | E-MEXP-1290 | biostudies-arrayexpress

Increased pro-inflammatory phenotype of peripheral blood monocytes and monocyte-derived macrophages among patients with excessive ethanol intake

Project description:Excessive alcohol consumption adversely affects the immune system and triggers the activation of peripheral blood (PB) monocytes and tissue macrophages, contributing to alcohol- related organ damage. This study aimed to analyze the M1/M2 and inflammatory phenotypes of circulating monocytes and macrophage-derived monocytes (MDMs). This single-center cross- sectional study included 20 excessive alcohol drinkers (EADs) and 22 healthy controls. PB samples were collected under fasting conditions for isolation of CD14+ monocytes and short-term culture without stimulation, LPS/IFNγ, or IL4/IL13. These conditions were also used to polarize monocyte-derived macrophages (MDMs) into M0, M1, or M2 phenotypes. Cytokine production was assessed in the blood samples and supernatants. M1/M2 related markers in PB monocytes and MDMs were analyzed using mRNA expression and surface marker analyses. In addition, the miRNA profile was analyzed in CD14+ monocytes. PB samples from EADs exhibited increased levels of pro-inflammatory cytokines.

2023-10-04 | GSE239659 | GEO

Transcriptomic profiling of cultured macrophages derived from bone marrow monocyte subsets

Project description:The bone marrow monocyte population contains subsets expressing differing amount of LFA-1 and Mac-1. The current analysis has profiled the macrophages derived from the LFA-1-hi Mac-1-hi subsets (MOhi2) and the LFA-1-lo Mac-1-lo subset (MOlo2). We found the transcriptome profiles of macrophages derived from MOhi2 (Machi2) differed from that of macrophages derived from MOlo2 (Maclo2). Machi2 and Maclo2 expressed different surface receptor markers expression and exhibited different panels of transcription factor activities. Functional enrichment analyses suggested Machi2 specializes in cell cycle related processes whereas Maclo2 has enriched expression of genes involved in immune responses.

2022-07-27 | GSE174721 | GEO

Periostin may act on monocytes and monocyte-derived macrophages with a pro-fibrotic phenotype in patients with systemic sclerosis (SSc)

Project description:Background: Circulating periostin levels are increased in systemic sclerosis (SSc) patients and correlates positively with disease severity. Additionally, monocytes/macrophages are one of the key cells in SSc pathogenesis. However, the effect of periostin on immune cells, particularly monocytes and macrophages, has not been well investigated. Objectives: To examine the effect of periostin on monocytes and monocyte-derived macrophages (MDMs) in SSc. Methods: Monocytes were purified from blood samples of nine diffuse cutaneous SSc patients and five healthy controls (HCs) and were differentiated into macrophages. Cell surface markers were assessed by flow cytometry. RNA sequencing analysis was performed on recombinant periostin-stimulated monocytes from three HCs. Cytokine/chemokine and extracellular matrix (ECM) protein expressions in MDMs differentiated supplemented with recombinant periostin were assessed by quantitative real-time polymerase chain reaction and bead-based immunoassays in six HCs. Moreover, we evaluated whether periostin was involved in monocyte migration by Transwell assay. Results: The modified Rodnan total skin thickness score correlated positively with the proportion of CD80-CD206+ M2 cells. The M2 macrophage proportion was reduced in rPn-stimulated MDMs in HCs, but not in SSc patients. mRNA expression levels of pro-fibrotic cytokine, chemokine, and ECM protein genes were significantly upregulated in recombinant periostin-stimulated monocytes and MDMs as compared to those from control monocytes and MDMs. Similar trends for their protein levels were also observed in MDMs. Moreover, the ratio of migrated cells in recombinant periostin-stimulated monocytes was significantly higher than that in control monocytes.

2022-09-22 | GSE213614 | GEO

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