Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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The overall effect of parasitization by Nasonia vitripennis on its host Sarcophaga crassipalpis


ABSTRACT: Nasonia vitripennis injects venom into its host organism Sarcophaga crassipalpis together with the eggs in order to make it suitable for the offspring to survive. The venom is known to suppress the hosts immune system, elevate the lipid levels, slow down development, et cetera.This microarray can uncover new transcriptomal effects on the host organism after natural envenomation that have not been discovered by bioassays. Since transcriptomal effects will vary during time, two different time points have been selected, 3 and 25 hours after parasitization. 16 individuals per sample, 4 replicates per group, loopdesign, 4 control individuals per time point, dye swap

ORGANISM(S): Sarcophaga crassipalpis

SUBMITTER: Wouter Van Delm 

PROVIDER: E-GEOD-36996 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Early changes in the pupal transcriptome of the flesh fly Sarcophagha crassipalpis to parasitization by the ectoparasitic wasp, Nasonia vitripennis.

Danneels Ellen L EL   Formesyn Ellen M EM   Hahn Daniel A DA   Denlinger David L DL   Cardoen Dries D   Wenseleers Tom T   Schoofs Liliane L   de Graaf Dirk C DC  

Insect biochemistry and molecular biology 20131023 12


We investigated changes in the pupal transcriptome of the flesh fly Sarcophaga crassipalpis, 3 and 25 h after parasitization by the ectoparasitoid wasp, Nasonia vitripennis. These time points are prior to hatching of the wasp eggs, thus the results document host responses to venom injection, rather than feeding by the wasp larvae. Only a single gene appeared to be differentially expressed 3 h after parasitization. However, by 25 h, 128 genes were differentially expressed and expression patterns  ...[more]

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