The transcriptional landscape of hematopoietic stem cell ontogeny

ABSTRACT: Transcriptome analysis of adult hematopoietic stem cells (HSC) and their progeny has informed our understanding of blood differentiation and leukemogenesis, but a similarly transformative analysis of the embryonic origins of hematopoiesis is lacking. To address this issue, we acquired gene expression profiles of developing HSC purified from over 2500 dissected murine embryos and adult mice, and applied a network biology-based analysis to reconstruct the gene regulatory networks of sequential stages of HSC development. We found that embryonic hematopoietic elements clustered into three distinct transcriptional states characteristic of the definitive yolk sac, HSCs emerging from hemogenic endothelium, and definitive HSCs. We functionally validated several candidate transcriptional regulator

ORGANISM(S): Mus musculus

SUBMITTER: Patrick Cahan 

PROVIDER: E-GEOD-37000 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress