Alkbh1/Tzfp Repress a Non-Repeat piRNA Cluster in Pachytene Spermatocytes

ABSTRACT: Piwi proteins and Piwi-interacting small RNAs (piRNAs) have known functions in transposon silencing in the male germline of fetal and newborn mice. Both are also necessary for spermatogenesis in adult testes, however, their function here remains a mystery. Here, we use germ cell isolations and small RNA sequencing to show that most piRNAs in meiotic spermatocytes originate from clusters in intergenic non-repeat regions of DNA. The regulation of these piRNA clusters, including the processing of the precursor transcripts into individual piRNAs, is accomplished through mostly unknown processes. We present evidence for a regulatory mechanism for one such cluster, named cluster 1082B, located on chromosome 7 in the mouse genome, containing 788 unique piRNAs. The precursor transcript and individ

ORGANISM(S): Mus musculus

SUBMITTER: Jonas Paulsen 

PROVIDER: E-GEOD-37150 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress