Dynamic binding of RBPJ is determined by Notch signalling status

ABSTRACT: Notch signalling plays crucial roles in mediating cell fate choices in all metazoans largely by specifying the transcriptional output of one cell in response to a neighbouring cell. The DNA-binding protein RBPJ is the principle effector of this pathway in mammals and together with the transcription factor moiety of Notch (NICD) it regulates the expression of target genes. The prevalent view presumes that RBPJ statically occupies consensus binding sites while exchanging repressors for activators in response to NICD. We present the first specific RBPJ chromatin immunoprecipitation and high-throughput sequencing study in mammalian cells. To dissect the mode of transcriptional regulation by RBPJ and identify its direct targets, whole genome binding profiles were generated for RBPJ, its coactiv

ORGANISM(S): Mus musculus

SUBMITTER: Arjen Brinkman 

PROVIDER: E-GEOD-37184 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress