HITS-CLIP analysis of FUS in lineage-committed cells.

ABSTRACT: In the present study, we performed HITS-CLIP analysis for FUS using mouse brain to extensively characterize tits RNA-binding sites and functional roles in RNA metabolisms. We identified preferential binding of FUS to stem-and-loop structures but without any discernible consensus motifs. FUS was preferentially bound to introns and 3' untranslated regions, but the exon/intron boundaries were mostly devoid of FUS-tags. Analysis of position-dependence of FUS-binding sites in regulating inclusion and skipping of exons disclosed that FUS is bound broadly around the alternatively spliced exons. Among them, however, noticeable CLIP-tags were observed in the downstream introns. We also noticed that FUS occasionally binds to the antisense strands in the promoter regions. Global analysis of CLIP-tags

ORGANISM(S): Mus musculus

SUBMITTER: Akio Masuda 

PROVIDER: E-GEOD-37190 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress