Identification of miR-21 targets in Jurkat cells by AGO2 immunoprecipitation

ABSTRACT: In order to identify miR-21 targets by a biochemical high-throughput method, we immunopurified RISC Complex and associated mRNAs in both control and miR-21 overexpressing Jurkat cells. Following transduction of miR-21 encoding construct (or a cognate control vector) in Jurkat cells, we used microarray technology to profile Total RNA, AGO2 Immunopurified RNA and control IgG purified RNA.

ORGANISM(S): Homo sapiens

SUBMITTER: Teresa Colombo 

PROVIDER: E-GEOD-37212 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress