Selective chemical labeling reveals the genome-wide distribution of 5-hydroxymethylcytosine in rice

ABSTRACT: We generated a genome-wide map of 5hmC in rice panicle using a sensitive chemical labeling method to capture DNA fragments with 5hmC modification, followed by NGS sequencing. We showed that the 5hmC peaks distribution is non-random, about 52% 5hmC was located in gene bodies, 25% in intergenic regions, 11% in promoters, and 12% in immediate downstream. It was significantly enriched in heterochromatic regions of chromosomes 4 and 10, and especially located around TE genes. Combined with RNA-seq transcriptome data, we found that the gene-specific alterations of 5hmC can result in gene activation or repression, suggesting a potential role for 5hmC in gene regulation. From the compared distribution analysis among super-hybrid rice cultivars LYP9, and its parental lines 93-11 and PA64s, we inves

ORGANISM(S): Oryza sativa

SUBMITTER: Shuhui Song 

PROVIDER: E-GEOD-37242 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress