Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Impact of Elevated Plasma 5-HT on Global Gene Expression of Murine Megakaryocytes

ABSTRACT: The goal of this project was to understand the functional consequences of plasma biogenic amine serotonin (5-HT) elevation in megakaryocytes by measuring gene expression Total RNA was extracted from the megakaryocytes of mice treated with either 5-HT or saline and global gene expression was measured.

ORGANISM(S): Mus musculus

SUBMITTER: fusun kilic

PROVIDER: E-GEOD-37273 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Impact of elevated plasma serotonin on global gene expression of murine megakaryocytes.

Mercado Charles P CP Byrum Stephanie S Beggs Marjorie L ML Ziu Endrit E Singh Preeti P Raj Vinay R VR Haun Randy S RS Kilic Fusun F

PloS one 20130827 8

<h4>Background</h4>Serotonin (5-HT) is a biogenic amine that also acts as a mitogen and a developmental signal early in rodent embryogenesis. Genetic and pharmacological disruption of 5-HT signaling causes various diseases and disorders via mediating central nervous system, cardiovascular system, and serious abnormalities on a growing embryo. Today, neither the effective modulators on 5-HT signaling pathways nor the genes affected by 5-HT signal are well known yet.<h4>Methodology/principal findi ...[more]

PMID: 24013211

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Project description:Hematopoiesis is a well-established model system to study molecular mechanisms of lineage-specific differentiation. Key transcription factors (TFs), such as PU.1, NF-E2 and GATA1 are implicated in crucial aspects of distinct hematopoietic lineages. How TFs collaborate with histone modificatons and how they affect the chromatin status remains to be elucidated. Chromatin-Immunoprecipitation followed by next-generation sequencing (ChIP-seq) has been proven to be an excellent tool to study chromatin modifications genome-wide. In this study, ChIP-seq was used to investigate the H3K4me2 landscape at enhancers during hematopoietic differentiation, starting from the hematopoietic stem cell (HSC) up to the fully differentiated erythrocyte, megakaryocyte or granulocyte. Although cell morphology and gene expression profiles differ extensively in committed erythrocytes, megakaryocytes and granulocytes, the genomic landscape of H3K4me2 is surprisingly alike across the three cell types. Granulocytes, in particular, seem to display an ‘erythocyte-like’ chromatin pattern. Similar results were observed for another chromatin mark, H3K27ac. Unexpectedly, the common progenitors of erythrocytes and granulocytes do not display a similar chromatin pattern, excluding the idea that the H3K4me2 mark is placed early on during differentiation. These results also suggest that the chromatin state is probably not the determining factor for lineage differentiation, but that rather lineage specific TF binding is important. In agreement with this, mature megakaryocytes loose the H3K4me2 mark surrounding erythrocyte-specific genes. This might be explained because megakaryocytes and erythrocytes both express the lineage specific TFs GATA1 and NF-E2. Altogether, we show that committed granulocytes and erythrocytes display similar H3K4me2 and H3K27ac patterns, and that those marks alone cannot predict which genes will be expressed eventually. Genomic ChIP-Seq on key transcription factors and histone modification marks in hematopoiesis

Dataset Information

Impact of Elevated Plasma 5-HT on Global Gene Expression of Murine Megakaryocytes

Publications

Impact of elevated plasma serotonin on global gene expression of murine megakaryocytes.

Similar Datasets

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