Tumorgenic properties of high-dose ionizing radiation of primary cancer-associated fibroblasts

ABSTRACT: Cancer-associated fibroblasts (CAFs) are acknowledged key determinants in the progression of cancer and thereby represent important targets for cancer therapies. Given the increase attention that ablative radiotherapy is gaining in the clinics, in this study we have aimed at identifying the transcriptional responses occurring in primary CAFs exposed to high-dose irradiation. Established primary cancer-associated fibroblasts (CAFs) obtained from non-small-cell lung cancer (NSCLC) patient material were irradiated with a single dose of 18 Gy and total RNA was isolated 24 hrs after treatment. Radiation-induced transcriptional alterations were investigated by gene expression analysis using genome-wide microarrays. Obtained results were verified by qRT-PCR of relevant genes. To confirm the data

ORGANISM(S): Homo sapiens

SUBMITTER: Chris Fenton 

PROVIDER: E-GEOD-37318 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress