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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Nanos2-associated mRNAs in the embryonic male gonad

ABSTRACT: To identify Nanos2-associated mRNAs, we performed microarray analysis of mRNAs coprecipitated with FLAG-tagged NANOS2 with male gonad from Tg-Nanos2 enhancer-3xFLAG-Nanos2-3'UTR at E14.5. Biological duplicates were examined at each sample.

ORGANISM(S): Mus musculus

SUBMITTER: Rie Saba

PROVIDER: E-GEOD-37718 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Nodal/activin signaling promotes male germ cell fate and suppresses female programming in somatic cells.

Wu Quan Q Kanata Kohei K Saba Rie R Deng Chu-Xia CX Hamada Hiroshi H Saga Yumiko Y

Development (Cambridge, England) 20121205 2

Testicular development in the mouse is triggered in somatic cells by the function of Sry followed by the activation of fibroblast growth factor 9 (FGF9), which regulates testicular differentiation in both somatic and germ cells. However, the mechanism is unknown. We show here that the nodal/activin signaling pathway is activated in both male germ cells and somatic cells. Disruption of nodal/activin signaling drives male germ cells into meiosis and causes ectopic initiation of female-specific gen ...[more]

PMID: 23221368

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Gene expression of mouse embryonic gonad

Project description:To elucidate the function of NANOS2 to regulate the transcriptome in embryonic male germ cells, we performed expression microarray analysis of the embyornic gonads of the Nanos2+/-, Nanos2-/- male and wild type female from E12.5 to E15.5. The Nanos2+/- and Nanos2-/- female gonads at E15.5 were analyzed as a negative control. Biological duplicates were examined at each stage, genotype, and sex for each experiment.

2013-02-06 | E-GEOD-37716 | biostudies-arrayexpress

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Project description:To investigate the biochemical function of NANOS2, we performed expression microarray analysis of the embyornic male gonad of Nanos2 hetero, Nanos2 KO, and Nanos2 KO_Tg 3M-CM-^WFLAG-tagged Nanos2-M-NM-^TN10, which is truncated form of Nanos2 in the N-terminal region, transgenic mice. 3M-CM-^WFLAG-tagged Nanos2-M-NM-^TN10 protein function was validated by rescue experiment in the Nanos2-null male gonad of mouse embryo at E14.5. Biological duplicates were examined at each genotype, Nanos2 hetero, Nanos2 KO, and Nanos2 KO_Tg 3M-CM-^WFLAG-tagged Nanos2-M-NM-^TN10 for each experiment.

2012-03-31 | E-GEOD-33138 | biostudies-arrayexpress

Gene expression of mouse embryonic gonad [Nanos2_Stra8]

Project description:To examine whether the meiosis was solo factor responsible for the disruption of the male gonocyte differenitiation in the Nanos2-/- male germ cells, we deleted Stra8 function from Nanos2-null background and performed expression microarray analysis of the Nanos2+/-/Stra8+/- , Nanos2-/-/Stra8+/-, and Nanos2-/-/Stra8-/- male emnbryonic gonads at E14.5. Biological duplicates were examined at each stage, genotype, and sex for each experiment.

2013-02-06 | E-GEOD-38294 | biostudies-arrayexpress

Role of Nanos2 in the embryonic male gonad

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-02-06 | E-GEOD-37720 | biostudies-arrayexpress

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Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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2013-04-01 | E-GEOD-38299 | biostudies-arrayexpress

Gene expression of mouse embryonic gonad [Cyp26b1]

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2013-02-06 | E-GEOD-38297 | biostudies-arrayexpress

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