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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse putative muscle derived stem cells

ABSTRACT: Comparison of NRA derived from SCA1+, CD45- MACS purified putative muscle derived stem cells vs. RNA derived from preplating of hind limb muscle cells. Experiment Overall Design: this experiment include 2 samples and 8 replicates

ORGANISM(S): Mus musculus

SUBMITTER: OGIC Info Ontario Genomics Innovation Centre (OGIC)

PROVIDER: E-GEOD-3773 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Putative muscle derived stem cells from mouse.

Project description:Comparison of NRA derived from SCA1+, CD45- MACS purified putative muscle derived stem cells vs. RNA derived from preplating of hind limb muscle cells. Keywords: other

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Transcription profiling of mouse Sca1+ primary myospheres isolated from Balb/c animals

Project description:Cells were isolated from muscle (hind leg) of Balb/c mouse (male). Then cells were enriched at FACS by using Sca1 antigen. Cells were grown 4 days in NSC medium, passaged and RNA isolated. Cells were at passage 1. Experiment Overall Design: this experiment include 1 samples and 6 replicates

2008-06-12 | E-GEOD-3235 | biostudies-arrayexpress

Gene expression profiling of CD45+/Sca1+ cells isolated from the bone marrow and the muscle

Project description:The BM-derived CD45+/Sca1+ cells are haematopoietic stem/progenitor cells that have the ability to circulate and migrate and engraft to the muscle tissue, and therefore they are of particular interest. Notably, these cells retain their haematopoietic potential, as revealed both by in vitro and in vivo assays; but they also acquire myogenic potential, as shown by their ability to participate in muscle regeneration. Whether, this latter remarkable ability is the result of the reprogramming of the BM-CD45+/Sca1+ cells and the activation of a myogenic molecular program within these cells, remains controversial. This study aims to clarify this aspect of the process, investigating the role of the muscle microenviroment and key myogenic transcription factors. Keywords: CD45+/Sca1+ cells, BM, muscle CD45+/Sca1+ cells isolated from the BM or the muscle were processed fresh and their RNA was extracted. Moreover, CD45+/Sca1+ cells isolated from the muscle of BM transplanted or untransplanted mice after injury with Cardiotoxin were processed fresh and their RNA was extracted.

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Gene expression profiling of CD45+/Sca1+ cells isolated from the bone marrow and the muscle

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Transcription profiling of mouse Sca1-CD45 sorted bone marrow

Project description:Sca1-CD45 sorted bone marrow Experiment Overall Design: this experiment include 4 samples and 22 replicates

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Proteome analysis of claws, clawless toe tips and back skin of Xenopus tropicalis

Project description:The aim of the study was to determine the protein composition of cornified claws of the western clawed frog (Xenopus tropicalis) in comparison to clawless toe tips and back skin. Cornified claws develop on toes I, II, III of the hind limbs, which we refer to as hind limb inner (HI) toes. Toes IV, V of the hind limbs, here referred to as hind limb outer (HO) toes lack claws. Proteins were prepared from HI toe tips including claws, HO toe tips and back skin (BSK) of frogs each (F1, F2, F3) and subjected to proteomic analysis.

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Transcription profiling of skeletal muscle from amyotrophic lateral sclerosis sod1(G86R) axotomized mice and control mice to monitor denervation-dependent gene expression in an Amyotrophic lateral sclerosis (ALS) mouse model

Project description:Amyotrophic lateral sclerosis (ALS) is a fatal adult-onset neuromuscular disorder characterized by the selective degeneration of upper and lower motor neurons, progressive muscle wasting and paralysis. To define the full set of alterations in gene expression in skeletal muscle during the course of the disease, we performed high-density oligonucleotide microarray analysis of gene expression in hind limb skeletal muscles of sod1(G86R) mice, one of the existing transgenic models of ALS. To monitor denervation-dependent gene expression, we determined the effects of short-term acute denervation on the muscle transcriptome after sciatic nerve axotomy.

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Expression data during plantaris muscle regrowth following two weeks of hind limb suspension in young adult mice

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2014-04-16 | E-GEOD-56798 | biostudies-arrayexpress

RNA-Seq transcriptome profiling of soleus from adult and aged mice following disuse muscle atrophy and recovery

Project description:Rodent hind limb unloading was used as a model for reduced muscle activity and eventual atrophy. After a 10 day period of unloading, mice in this study were “reloaded” for 3 days and regained use of their hind limbs. We report the application of Next-generation sequencing (NGS) technology for high-throughput profiling of mRNA in soleus muscle of adult (6 mo) and aged (22-24 mo) mice. Our goal was to determine the effects of hind limb unloading and reloading on mRNA profiles in soleus muscle and compare between adult and aged mice. We find that there are distinct response in the profile of fatty acid oxidation, TCA cycle, ETC oxidative phosphorylation gene expression patterns in response to unloading and reloading. The repsonses are generally simialr between young and old mice.

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Sca1+ primary myospheres

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