Transcription profiling of rat single cell derived calvaria cell colonies with adipocyte and bone markers

ABSTRACT: Single cell-derived colonies were obtained by sorting 21 days fetal Rat calvaria cells into microtitre wells using flow cytometry. Colonies were categorized according to a limited gene expression profiling for a specific bone (osteocalcin) and a specific adipocyte (adipsin) marker by real-time PCR. Colonies expressing neither, one or both bone and adipocyte markers are in the sample set. Microarray analysis will address the global gene expression profiles of CFU-O (bone marker only), CFU-F (neither bone nor adipocyte marker) and CFU-bipotential (both markers) derived from single cells. CFU-A (adipocyte marker only) samples are also available for analysis if warranted. Experiment Overall Design: this experiment include 3 samples and 18 replicates

ORGANISM(S): Rattus norvegicus

SUBMITTER: OGIC Info Ontario Genomics Innovation Centre (OGIC) 

PROVIDER: E-GEOD-3775 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress