Differentiation of Human Embryonic Stem Cells under Hypoxia Conditions

ABSTRACT: Early development of mammalian embryos occurs in an environment of relative hypoxia. Nevertheless, human embryonic stem cells (hESC), which are derived from the inner cell mass of blastocyst, are routinely cultured under the same atmospheric conditions (21% O2) as somatic cells. We hypothesized that O2 levels modulate gene expression and differentiation potential of hESC, and thus, we performed gene profiling of hESC maintained under normoxic or hypoxic (1% or 5% O2) conditions. Our analysis revealed that hypoxia downregulates expression of pluripotency markers in hESC but increases significantly the expression of genes associated with angio- and vasculogenesis including vascular endothelial growth factor and angiopoitein-like proteins. Consequently, we were able to efficiently differentia

ORGANISM(S): Homo sapiens

SUBMITTER: david montaner 

PROVIDER: E-GEOD-37761 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress