Inferring gene regulatory logic from high-throughput measurements of thousands of systematically designed promoters

ABSTRACT: Despite extensive research, our understanding of the rules according to which cis-regulatory sequences are converted into gene expression is limited We devised a method for obtaining parallel, highly accurate gene expression measurements from thousands of designed promoters and applied it to measure the effect of systematic changes in the location, number, orientation, affinity and organization of transcription-factor binding sites and nucleosome-disfavoring sequences. Our analyses reveal a clear relationship between expression and binding-site multiplicity, as well as dependencies of expression on the distance between transcription-factor binding sites and gene starts which are transcription-factor specific, including a striking ~10-bp periodic relationship between gene expression and bin

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Eilon Sharon 

PROVIDER: E-GEOD-37851 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress