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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human and mouse first passage vs. primary retinal sphere cells

ABSTRACT: The pigmented portion of ciliary epithelium in the adult mammalian eye harbors mitotically quiescent retinal sphere cells, which are capable of self-renewal and differentiating into retinal neurons when assayed in vitro; however, very little is known about the molecular mechanism controlling the proliferation and differentiation of these adult retinal stem cells or their molecular resemblance to mutipotent stem/progenitor cells during early eye development. This experiment studies the gene expression of first passage and primary human and mouse retinal sphere cells. Experiment Overall Design: this experiment include 4 samples and 12 replicates

ORGANISM(S): Homo sapiens

SUBMITTER: OGIC Info Ontario Genomics Innovation Centre (OGIC)

PROVIDER: E-GEOD-3791 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene Expression Comparison of First Passage vs. Primary Human and Mouse Retinal Sphere Cells

Project description:The pigmented portion of ciliary epithelium in the adult mammalian eye harbors mitotically quiescent retinal sphere cells, which are capable of self-renewal and differentiating into retinal neurons when assayed in vitro; however, very little is known about the molecular mechanism controlling the proliferation and differentiation of these adult retinal stem cells or their molecular resemblance to mutipotent stem/progenitor cells during early eye development. This experiment studies the gene expression of first passage and primary human and mouse retinal sphere cells. Keywords: other

2005-12-09 | GSE3791 | GEO

Transcription profiling of mouse sorted bone marrow samples to determine genes implicated in hematopoetic stem cell identity

Project description:Gene expression profiles of sorted bone marrow samples to determine genes implicated in hematopoetic stem cell identity. Experiment Overall Design: this experiment include 6 samples and 32 replicates

2008-06-12 | E-GEOD-3234 | biostudies-arrayexpress

Transcription profiling of mouse mammary epithelial stem cells and differentiated derivatives

Project description:Gene Expression Profiling of Murine Mammary Stem Cells and Differentiated Derivatives.

2007-12-15 | E-GEOD-3711 | biostudies-arrayexpress

Transcription profiling of mouse pluripotet embyonal carcinoma cells

Project description:Investigation of very early events required for initiating differentiation of pluripotent embryonal carcinoma cells - the effect of cell aggregation on gene expression. Experiment Overall Design: this experiment include 4 samples and 24 replicates

2008-06-12 | E-GEOD-4085 | biostudies-arrayexpress

Gene Expression Comparison of First Passage vs. Primary Human and Mouse Retinal Sphere Cells

Project description:Gene Expression Comparison of First Passage vs. Primary Human and Mouse Retinal Sphere Cells

| PRJNA93989 | ENA

Transcription profiling of mouse undifferentiated and differentiated mammospheres isolated from FVB animaks

Project description:Mammary gland cells were isolated from 5-8 week old FVB mice and allowed to proliferate as undifferentiated mammospheres in the presence of growth factors (bFGF and EGF). Comparison of the gene expression profiles of undifferentiated mammospheres vs. mammospheres that were allowed to differentiate for 6 days by the removal of growth factors will help to identify genes that are implicated in the maintenance of the mammary gland stem cell compartment. Experiment Overall Design: this experiment include 2 samples and 12 replicates

2008-06-12 | E-GEOD-3777 | biostudies-arrayexpress

Transcription profiling of mouse pRb null primary myoblasts and myotubes will provide a global picture of the downstream targets of pRb transcriptional regulation in relation to cell cycle control, apoptosis inhibition, and muscle differentiation

Project description:Satellite cells play an important role in post-natal growth and regeneration of skeletal muscle. They can be defined as a population adult muscle stem cells based on their self renewal capability and ability to differentiate into skeletal muscle fibers. Functional Retinoblastoma protein (pRb) is essential for the process of skeletal muscle differentiation in satellite cell derived primary myoblasts. Furthermore, the biochemical function of pRb is largely associated with its ability to interact with chromatin modifying factors such as histone deacetylases (HDACs) and histone methyltransferases thus inhibiting transcription of target gene promoters. Hence, expression profiling of pRb null primary myoblasts and myotubes will provide a global picture of the downstream targets of pRb transcriptional regulation in relation to cell cycle control, apoptosis inhibition, and muscle differentiation. Experiment Overall Design: This experiment includes 4 samples with 3 replicates each on 2 platforms for a total of 24 Samples.

2008-04-08 | E-GEOD-10428 | biostudies-arrayexpress

Form deprivation modulates retinal neurogenesis in primate experimental myopia

Project description:Juvenile primates develop myopia when their visual experience is degraded by lid fusion. In response to the abnormal visual input, retinal neural networks cause an excessive growth of the postequatorial segment of the eye, but the mechanism underlying this axial elongation is unknown. By combining analysis of gene expression, injection of the thymidine analog 5-bromo-2'-deoxyuridine and immunocytochemistry, we show that the retinal periphery in both juvenile rhesus macaques and green monkeys harbors a population of mitotically active neuroprogenitor cells that proliferate when the visual experience is altered by lid fusion. Furthermore, the number of dividing cells is highly correlated with the axial elongation of the eye and the resulting myopic refractive error. Thus, the retina undergoes active growth during the postnatal development of the primate eye. This growth is modulated by the visual input and accelerates considerably when the eye develops axial myopia. Keywords: disease state analysis

2006-02-27 | GSE3300 | GEO

Chondrosarcoma cells: sphere vs adherent

Project description:Transcriptional profiling of human chondrosarcoma sphere vs adherent cells Two different growth conditions, sphere vs adherent

2015-04-30 | E-GEOD-47823 | biostudies-arrayexpress

Transcription profiling of rat single cell derived calvaria cell colonies with adipocyte and bone markers

Project description:Single cell-derived colonies were obtained by sorting 21 days fetal Rat calvaria cells into microtitre wells using flow cytometry. Colonies were categorized according to a limited gene expression profiling for a specific bone (osteocalcin) and a specific adipocyte (adipsin) marker by real-time PCR. Colonies expressing neither, one or both bone and adipocyte markers are in the sample set. Microarray analysis will address the global gene expression profiles of CFU-O (bone marker only), CFU-F (neither bone nor adipocyte marker) and CFU-bipotential (both markers) derived from single cells. CFU-A (adipocyte marker only) samples are also available for analysis if warranted. Experiment Overall Design: this experiment include 3 samples and 18 replicates

2008-06-12 | E-GEOD-3775 | biostudies-arrayexpress

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