Constitutive activation of the receptor-like kinase NIK impairs translation and confers tolerance to begomovirus infection

ABSTRACT: To generate an efficient defense against begomovirus, we modulated the activity of the immune defense receptor NIK (NSP-Interacting Kinase) in tomato plants; NIK is a virulence target of the begomovirus NSP during infection. Replacing threonine-474 with aspartate (T474D) within the kinase activation loop promoted the constitutive activation of NIK-mediated defenses. This activation resulted in the down-regulation of translation-related genes and the suppression of global translation in T474D-overexpressing tomato lines. We also found that T474D-induced defense-related transcripts were associated with polysomes and immune proteins, which accumulated to detectable levels in T474D leaves. Consistent with these findings, T474D transgenic lines were tolerant to the tomato-infecting begomoviruse

ORGANISM(S): Solanum lycopersicum

SUBMITTER: Elizabeth Fontes 

PROVIDER: E-GEOD-38028 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress