Co-culture of human hematopoietic stem cells with osteoblasts affects mono/macrophage versus erythroid lineage choice.

ABSTRACT: Hematopoietic stem cells (HSCs) are located in the bone marrow in a specific microenvironment referred as the hematopoietic stem cell niche, where HSCs interact with a variety of stromal cells. Though several components of the stem cell niche have been identified, the regulatory mechanisms through which such components regulate the stem cell fate are still unknown. In order to address this issue, we investigated how osteoblasts (OBs) can affect the molecular and functional phenotype of HSCs and vice versa. Our data showed that CD34+ cells cultured with OBs give rise to higher total cell numbers, produce more CFU and maintain a higher percentage of CD34+CD38- cells compared to control culture. Moreover, clonogenic assay and long-term culture results showed that OBs enhance HSC differentia

ORGANISM(S): Homo sapiens

SUBMITTER: Rossella Manfredini 

PROVIDER: E-GEOD-38091 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress