Guthrie card methylomics identifies temporally stable epialleles that are present at birth in humans (Bisulfite-Seq)

ABSTRACT: A major concern in common disease epigenomics is distinguishing causal from consequential epigenetic variation. One means of addressing this issue is to identify the temporal origins of epigenetic variants via longitudinal analyses. However, prospective birth-cohort studies are expensive and time-consuming. Here we report DNA methylomics of archived Guthrie cards for the retrospective longitudinal analyses of in utero-derived DNA methylation variation. We first validate two methodologies for generating comprehensive DNA methylomes from Guthrie cards. Then, using an integrated epigenomic/genomic analysis of Guthrie cards and follow-up samplings, we identify inter-individual DNA methylation variation that is present both at birth and three years later. These findings suggest that disease-rel

ORGANISM(S): Homo sapiens

SUBMITTER: Thomas Down 

PROVIDER: E-GEOD-38127 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress