Aspergillus nidulans WT, M-NM-^TcrzA and M-NM-^TcrzA/scrA-: Control vs. calcium pulse

ABSTRACT: The main objective was to identifiy genes related to scrA- extragenic suppression of crzA. Thus, through transcriptional profiling, we characterised the transcriptional basis of calcium tolerance in our mutant strain, M-NM-^TcrzA/scrA-. To evaluate the effect of calcium on global A. nidulans gene expression, we performed competitive microarray hybridizations using RNA obtained from the wild-type, M-NM-^TcrzA and M-NM-^TcrzA/scrA- strains before and after short pulses (10 and 30 minutes) of 200 mM calcium chloride. The experiment was further validated by real-time PCR. Two-condition experiment : A. nidulans wild-type, M-NM-^TcrzA and M-NM-^TcrzA/scrA- strains with 200 mM CaCl2 for 10 and 30 min at 30M-BM-0C and 150 rpm in 25 mL YAG medium. Firstly, the strains were grown overnight at 30M-BM

ORGANISM(S): Emericella nidulans

SUBMITTER: Wagner de Souza 

PROVIDER: E-GEOD-38131 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress